Liz,

We have used the Dako Mast Cell Tryptase, clone AA1, for several years
with great results. It works on formaldehyde fixed paraffin sections
following proteolytic pre-treatment, it also works on Carnoy fixed
sections without pre-treatment.

Bryan 

>----------
>From: 	Liz Sabin[SMTP:esabin@srv4.med.ed.ac.uk]
>Sent: 	May 20, 1999 9:11 AM
>To: 	Histonet@Pathology.swmed.edu
>Subject: 	Mast Cells
>
>Hi all,
>Can anyone suggest an antibody to human Mast Cells which will work on 
>paraffin sections?  The only one I've seen is to Mast Cell Tryptase, 
>which both Dako and Novocastra sell.  Has anyone had any experience 
>with these antibodies?  Any hints/tips?
>Thanks,
>Best wishes,
>Liz Sabin.
>
>
>
>
>
>
>Liz Sabin
>Department of Pathology
>University of Edinburgh
>Teviot Place
>Edinburgh
>EH8 9AG
>
>Tel. 0131 650 2960
>Fax. 0131 650 6528
>
>