Barb,
I have used the Signet USA kit and have had good results even with
antibodies like CD-15 (IgM class)

> -----Original Message-----
> From:	Terrett, Barb [SMTP:Barb_Terrett@pmh.toronto.on.ca]
> Sent:	Monday, May 31, 1999 8:34 AM
> To:	'histonet@pathology.swmed.edu'
> Subject:	IgG vs IgM and Signet ultrastreptavidin
> 
> Good morning histonetters!
> I have a question for anyone using the Signet Ultra Streptavidin detection
> system reagents. Most of the Ab's we use are of the IgG chain composition,
> however, a few are of the IgM chain composition e.g. CD15 and sarcomeric
> actin. Right now we use a separate link for the IgM Ab's, but I was
> wondering if the signet link is o.k. to use with the IgM Ab's. This would
> safe some hassle. Does anyone have experience with this, or suitable
> references?
> Thanks for your time
> ttfn b :)