IgG vs IgM and Signet ultrastreptavidin

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From:"Terrett, Barb" <Barb_Terrett@pmh.toronto.on.ca>
To:"'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu>
Date:Mon, 31 May 1999 09:33:50 -0400

Good morning histonetters!
I have a question for anyone using the Signet Ultra Streptavidin detection
system reagents. Most of the Ab's we use are of the IgG chain composition,
however, a few are of the IgM chain composition e.g. CD15 and sarcomeric
actin. Right now we use a separate link for the IgM Ab's, but I was
wondering if the signet link is o.k. to use with the IgM Ab's. This would
safe some hassle. Does anyone have experience with this, or suitable
Thanks for your time
ttfn b :)

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