Edward,
I received some literature from Biocare Medical on CD-15.  They have a
monoclonal cocktail for CD-15 (clones DT07 + BC97).  You might give that a
try.  Their number is 1-800-799-9499. I haven't tried it yet, but my
understanding is that it works well in formalin-fixed tissue.  I'm using the
B-D CD15 on my Dako autostainer at a titer of 1:100 with a 30 minute
incubation.  Most of the tissue we have is B5-fixed. Hope this helps

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 


> -----Original Message-----
> From:	Edward Henry [SMTP:HenryE@holycrosshealth.org]
> Sent:	Monday, May 10, 1999 3:15 PM
> To:	histonet@Pathology.swmed.edu
> Subject:	CD15 antibody
> 
> Currently we are using DAKO predilutes on the DAKO immuno stainer with
> WONDERFUL results.  However, as fate would have, our Hempath wants to
> do CD15 using a non-clone.  DAKO only supplies the clone.  Does anyone
> (using the DAKO) have a technique and a supplier for the CD15 antibody
> that you would not mind sharing with us?
> 
> Better yet, is anyone using the Becton-Dickinson CD15 on the DAKO
> stainer?
> 
> Edward Henry, Manager
> Anatomic Pathology
> Holy Cross Hospital
> Silver Spring, MD
> henrye@holycrosshealth.org