All in all the practice of "changing the angle to match the block you have to recut as needed" is not a good practice.
During it you could end loosing tissue when trying to "build" a new flat surface from a block that was originally cut at a different angle, while "sculping" the new surface.
The best practice is to have all the microtomes cutting at the same angle and try to convince those HTs that say that "I have always cut with this angle" that they are flat wrong and a general angle can be good for the majority of blocks.
Amos Brooks wrote:
Histonet mailing list