Thanks for your advice - I've just seen your other messgae on Histonet
(just back from hols so still catching up). I found one ref where they
immersed the tissue in PLP, however in this case they then dehydated and
embedded in paraffin. Cancer Immuno Immunother 2005 vol 54 pge229. I
dont have easy access to paraffin embedding as I always work on frozen
tissue so I'll try the immersion and freezing - if that doesnt work I'll
do the perfusion.
Will let you all know if I have any success!
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