RE: [Histonet] IHC antibodies for animals

From:"Patsy Ruegg"

I used the Lab Vision polyclonal CD20.

-----Original Message-----
From: Jan Shivers [] 
Sent: Monday, May 07, 2007 3:01 PM
To: Patsy Ruegg
Subject: Re: [Histonet] IHC antibodies for animals

Hi Patsy,

Could you tell me which CD20 from Lab Vision you use?  I see they have one 
polyclonal and three monoclonal CD20s.  I'm in a search for another B cell 
marker, since Dako discontinued selling B Lymphocyte Antigen 36.

Thanks so much for any help you can provide.
Jan Shivers
U of MN Vet Diag Lab

----- Original Message ----- 
From: "Patsy Ruegg" 
To: "'Patricia Adams'" ; "'HistoNet'" 

Sent: Monday, May 07, 2007 3:16 PM
Subject: RE: [Histonet] IHC antibodies for animals

> Patricia,
> I have used CD20 from Lab Vision on dogs, this does not require any
> pretreatment AR.  Also used cd79a from Dako for B cells on dog tissue. 
> For
> T cells I highly recommend Lab Vision's rab monoclonal CD3, it works great
> with citrate HIER on dogs.
> Patsy
> -----Original Message-----
> From:
> [] On Behalf Of Patricia
> Adams
> Sent: Monday, May 07, 2007 1:45 PM
> To: HistoNet
> Subject: [Histonet] IHC antibodies for animals
> Hello all!
> Now that I am working with animals, I am looking
> for antibodies that react on all species. But
> right now canine is the most important.
> I used a CD20 and a CD43, human tonsil as the
> control.  Of course my controls were beautiful,
> but the canine pt. did not work and it should
> have.
> CD20 and CD43 were all the choices I had to pick
> for B-cell and T-cell.
> Could you please give me some advice?  We have a
> very small budget and I do not have the choice of
> buying antibodies just to see if they will work
> on animals.
> Thank you very much,
> Patricia Adams
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