We used the Abcam mouse antibody, clone 236A/E7 we great success on
both human cryo and FFPE samples, and also in double staining with
CD25, CD4 (cryo). For more details please see our paper recently
accepted for JHC: Onno J. de Boer et al., Immunohistochemical Analysis
of Regulatory T Cell Markers FOXP3 and GITR on CD4^+CD25^+ T Cells in
Normal Skin and Inflammatory Dermatoses. Go to the JHC website
(www.jhc.org) and go to exPRESS. You will find our paper under: May
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Mon, 7 May 2007 10:35:52 -0700
From: "Melissa Gonzalez"
Subject: [Histonet] Regulatory Tcells (FoxP3) IHC
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Anyone have any experience with this? How well does the Abcam antibody
work on FFPE human tissues? What's a good positive control?
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