Last year I tried an antibody from Cedarlane Leishmania LPG, clone
CA7AE (1:500, 60 min, RT). I could make it work with HIER citrate
pH6.0 and indirect fluorescence on FFPE samples. Spectral imaging was
used for unmixing "real" signal from autofluorescence. Even then it
was hard to find the specific signal, due to some specific-looking
nuclear background staining. The tissue samples that were positive
with fluorescence were also subjected to IHC with anti-mouse polymers
and DAB or LPR as chromogens. This totally failed and we gave up.
I realize it's not a very hopeful story but perhaps it helps anyway.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Mon, 7 May 2007 15:03:28 -0500
From: "Drew Sally A."
Subject: [Histonet] Leishmania
Could someone direct us to a place/person who might run an antibody
against Leishmania? We
have a pathologist asking questions about it, and all our usual source
don't list it.
Sally Ann Drew, MT(ASCP)
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
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