[Histonet] RE: Leishmania

From:"C.M. van der Loos"

   Dear Sally,

   Last  year  I  tried  an antibody from Cedarlane Leishmania LPG, clone
   CA7AE (1:500,  60  min,  RT).  I  could make it work with HIER citrate
   pH6.0 and indirect  fluorescence on FFPE samples. Spectral imaging was
   used for  unmixing  "real"  signal from autofluorescence. Even then it
   was  hard  to  find  the specific signal, due to some specific-looking
   nuclear  background  staining.  The  tissue samples that were positive
   with  fluorescence were also subjected to IHC with anti-mouse polymers
   and DAB or LPR as chromogens. This totally failed and we gave up.

   I realize it's not a very hopeful story but perhaps it helps anyway.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   Date: Mon, 7 May 2007 15:03:28 -0500
   From: "Drew Sally A." 
   Subject: [Histonet] Leishmania
   To: "Histonet" 
   Could someone direct us to a place/person who might run an antibody
   against Leishmania?  We
   have a pathologist asking questions about it, and all our usual source
   don't list it.
   Thank you!
   Sally Ann Drew, MT(ASCP)
   IHC/ISH Laboratory
   University of Wisconsin Hosp. & Clinics
   Madison, WI 53792
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