Re: [Histonet] Breast Marker Question

From:Patti Loykasek

Laura, hi. We do quite a bit of that type of determination here. We have
found that antibodies to smooth muscle myosin heavy chain and p63 are the
best markers. SMMHC is cytoplasmic & P63 is nuclear - sometimes on a case
one will look better than the other. It's nice as a double stain, too.  We
recently tested maspin and p-cadherin, but in our testing they were
inferior. Antibodies to calponin can be used, but that is much less

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

> What are the top two best markers for labeling the basement membrane
> components and myoepithelial cells of breast tissue (or maybe not the
> myoepithelial cells)?  We're looking for antibodies that would assist in
> differentiating between invasive and non-invasive breast carcinomas. One of
> our pathologists wants Laminin and Collagen Type IV, but the article I found
> was real old.  Are these two antibodies possibilities or are labs using other
> more sensitive and/or specific markers.
> Thanks,
> Laura
> Laura Bliven
> Histology Lab/IHC
> Marshfield Laboratories
> 1000 N. Oak Ave.
> Marshfield, WI 54449
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