RE: [Histonet] Large sections

From:"Michael J. Lyon, Ph.D."


We have in the past done serial sections of human larynges, both cross and
longitudinal.  They were formalin fixed, decalcified in formic acid and
paraffin embedded.  I would say the there would be some structural damage
due to the freezing in saline and it would be tough to get good frozen
sections since the thyroid cartilage may be calcified as it is in humans. 

What is your client truly after???


Michael J. Lyon, Ph.D.
Associate Prof Otolaryngology
SUNY Upstate Med. Univ.

> -----Original Message-----
> From: Patsy Ruegg []
> Sent: Tuesday, May 02, 2006 12:53 PM
> To:
> Subject: [Histonet] Large sections
> Can anyone suggest a resource for getting frozen sections of Elk Larynx?
> One of my clients asks:
> "I study elk sound production. As one question we investigate the
> anatomical
> structure of the larynx. For this purpose I need cross sections of several
> larynges (approx. 15 larynges). Cross sections at three positions are
> necessary for each larynx,. The larynges are most likely calcified at
> varying degrees. I have stored the larynges frozen in saline solution."
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email
> web site 
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