Stephanie U Gayle:

The old triangle glass knife makers (LKB, EBS, DuPont)
have a number of mechanical issues that need to be
addressed by the user--especially if you are having a
low percentage of good knives.  First, when was the
last time you changed the scoring wheel?  That would
be my first step.  Second, would be the damping pads
and it probably wouldn't hurt to replace them.  Next,
go through a check of the procedure following the
manual to evaluate the scoring pressure (follow the
user's manual as you do this).  Next, check the length
of the score--is it as described by the manual? 
Finally, (and this is a very personal thing) I use a
slow break method (i.e. turn the pressure lever/wheel)
just until you see the glass begin to fracture, then
let it fracture on its own.  I have had several LKB,
Leica and Dupont knifemakers over the years.  These
procedures have always results in a high percentage of
good knives.  Oh yes.  Be very careful when you pick
up the knives--if they touch at that point, then
they're probably no good!  

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
Ridgefield CT

--- gayle brosnanwatters
 wrote:

> I use glass knives (triangles) that I make from 1/2
> inch glass on an
> ancient knife maker that I play heck keeping
> operating. I, too, am
> frustrated by how hard it is to make decent knives. 
> I section plastic
> embedded mouse brains at between 1 and 3 microns and
> do light
> microscopy.  Even when my knives are working well, I
> can expect to use
> two or three per mouse brain, in order to get the
> sections I want.  My
> glass pieces are not the long strips of 1/4 inch
> pieces (although I have
> used them), but shorter rectangles.  I think if you
> can get your knife
> maker working well, it can make a difference.  I
> just had mine sent off
> to Energy Beam Sciences, and they got it working,
> but then they shipped
> it back to me and I really think that the shipper
> dropped it on the way,
> because it has not been working well since.  I
> understand your
> frustration, although I don't have an answer.  I
> wondered if the fact
> that my glass is old would make a difference.  I
> know just enough
> chemistry to remember that glass is a liquid that
> does change form as it
> ages, and this glass was given to me and was old
> then.  Does anyone know
> if that would affect it?
> Gayle L. Brosnan-Watters, PhD
> Assistant Professor
> Dept of Psychology
> Treasurer, Faculty for Undergraduate Neuroscience
> 226 Vincent Science Hall
> Slippery Rock University
> Slippery Rock, PA 16057
> gayle.brosnanwatters@sru.edu
> 724-738-2529 - office
> 724-738-4807 - fax
> 
> >  I make and use glass knifes for plastic
> sectioning
> > at 3 microns. I usually have to go through a whole
> > glass strip to get one good knife. Does anyone
> make
> > them anymore and have the same problem?
> > 
> > 
> > ~Stephanie Brusig
> > 
> > Weyerhaeuser Company
> > Propagation of High Value Trees
> > 32901 Weyerhaeuser Way S
> > WTC-1B10
> > Federal Way, WA 98001
> > 253.924.6518
> > 
> > 
> > -----Original Message-----
> > From: histonet-bounces@lists.utsouthwestern.edu
> > [mailto:histonet-bounces@lists.utsouthwestern.edu]
> > On Behalf Of
> > histonet-request@lists.utsouthwestern.edu
> > Sent: Wednesday, May 03, 2006 10:10 AM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: Histonet Digest, Vol 30, Issue 3
> > 
> > Send Histonet mailing list submissions to
> > 	histonet@lists.utsouthwestern.edu
> > 
> > To subscribe or unsubscribe via the World Wide
> Web,
> > visit
> > 
> >
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > or, via email, send a message with subject or body
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> > 
> > When replying, please edit your Subject line so it
> > is more specific than "Re: Contents of Histonet
> > digest..."
> > 
> > 
> > Today's Topics:
> > 
> >    1. CONTROL BLOCKS NEEDED (Nymeyer, Heather)
> >    2. Large sections (Patsy Ruegg)
> >    3. Autostainer tubes  (Patti Loykasek)
> >    4. NSH Active Members (Carrie Diamond)
> >    5. Question (Nita Searcy)
> >    6. Re: Question (Victoria Baker)
> >    7. Re: Large sections (Gayle Callis)
> >    8. RE: Question (Allen, Rhonda)
> >    9. RE: Question (Bartlett, Jeanine
> (CDC/NCID/VR))
> >   10. Re: Autostainer tubes (Joanne Mauger)
> >   11. Re: POC for Genetic Studies
> > (histology@gradymem.org)
> >   12. antibodies to eIF2 alpha and PPAR gamma
> (Sarka
> > Lhotak)
> >   13. IHC on frozens (Sarka Lhotak)
> >   14. Fwd: [Histonet] CD Abs (Linresearch@aol.com)
> >   15. gallocyanin stain for nissel  (Elizabeth
> > Chlipala)
> >   16. RANK, ER and PR in mouse (Randolph-Habecker,
> > Julie)
> >   17. NADH staining (Yak-Nam Wang)
> >   18. Re: gallocyanine stain for Nissl (John
> > Kiernan)
> >   19. Inflamatory markers (CRAIG BARLOW)
> >   20. Re- processing fatty tissues (Malam
> > Jacqueline)
> >   21. The end of an era (McGovern, Kevin)
> >   22. Mycobacterium bovis (BCG) antibody (Martha
> > Ward)
> >   23. Glycerol cryoprotected tissue and cryostat
> > sectioning
> >       (Kathie A Berghorn)
> >   24. RE: IHC on frozens (Guillermo Palao)
> >   25. Save These Dates For The Pennsylvania State
> > Histology	Meeting
> >       (Pamela Marcum)
> >   26. RE: Mycobacterium bovis (BCG) antibody
> > (Elizabeth Chlipala)
> > 
> > 
> >
>
----------------------------------------------------------------------
> > 
> > Message: 1
> > Date: Tue, 2 May 2006 10:03:23 -0700
> > From: "Nymeyer, Heather"
> > 
> > Subject: [Histonet] CONTROL BLOCKS NEEDED
> > To: 
> > Message-ID:
> > 
> >
>
<463911DE3F9F8D4AA3EC67632386BC62D1509E@dc1serv78.interiorhealth.ca>
> > Content-Type: text/plain;	charset="us-ascii"
> > 
> > We are in need of the following control blocks
> > 
> >             - Leprosy bacilli
> > 
> >             - Spirochete 
> > 
> >  
> > 
> > Your assistance is appreciated and thank you in
> > advance.
> > 
> >  
> > 
> > Heather D. Nymeyer, RT, CEBT
> > 
> > Charge Technologist, Anatomic Pathology
> > 
> > Royal Inland Hospital,
> > 
> > Kamloops, BC.
> > 
> > 250-314-2664
> > 
> >  
> > 
> > 
> > 
> > ------------------------------
> > 
> > Message: 2
> > Date: Tue, 2 May 2006 10:52:50 -0600
> > From: "Patsy Ruegg" 
> > Subject: [Histonet] Large sections
> > To: 
> > Message-ID:
> > <200605021652.k42Gqoog014137@chip.viawest.net>
> > Content-Type: text/plain;	charset="US-ASCII"
> > 
> > Can anyone suggest a resource for getting frozen
> > sections of Elk Larynx?
> >  
> > One of my clients asks:
> > "I study elk sound production. As one question we
> > investigate the anatomical structure of the
> larynx.
> > For this purpose I need cross sections of several
> > larynges (approx. 15 larynges). Cross sections at
> > three positions are necessary for each larynx,.
> The
> > larynges are most likely calcified at varying
> 
=== message truncated ===


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