Re: [Histonet] Mercurochrome in IBF--replacement?

From:"Vinnie Della Speranza"

Many laboratories will place a small quantity of eosin dye powder or eosin stain solution in the last alcohol on their processors. the specimen will be readily visible at the embedding station and the eosin is removed duirng staining (when you blue your hematoxylin) so your H&Es will not look differently.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>>  06/09/05 01:21PM >>>
I've read many articles in the archives about replacing mercurochrome with eosin, etc.  We are looking for a dye solution to use in IBF fixative for our prostate biopsies.  (In order to see the tissue easier at embedding).  We are currently trying Mrs. Stewart's bluing solution. With this, the pathologists have noticed a blue halo around the sections. Generally it does not interfere with diagnosis, but am wondering if there's something better out there?

Thank you in advance,

Jacqueline Farnsworth
Anatomic Pathology Tech III
Calgary Laboratory Services 
PH: (403) 944-1578
Pager: 212-8223 #1933

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