From:"McGraw, Medea J"

I am looking for information on fixing brain tissue to give the best detail
of tissue elements. I have read that Ammonium Bromide is currently using for
fixing brain tissue (helps detail the astrocytes). My question is: can you
post fix a brain that has already been fixed in 10% NBF with Ammonium
Bromide or do you have to put the fresh tissue directly into the Ammonium
Bromide? Any advice would be greatly appreciated.

Medea J. McGraw , B.S. HT (ASCP) 
SCCA Pathology Department
Desk: 206-288-1358
Main Lab: 206-288-1355
Email: mmcgraw@seattlecca.org

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