I need your advice on blocking. The animal was injected with human antibody. After the tissue are havested I have to do IHC using the same human antibody. I know the secondary antibody will pick up the antibody that is injected as well as the one I applied as primary antibody in IHC. I hope I said it clearly. So please tell me what kind of blocking I can use to block the injected human antibody? And how? Will normal human serum work?
Any input will be highly appreciated!
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