[Histonet] blocking

From:wen eng

I need your advice on blocking. The animal was injected with human antibody. After the tissue are havested I have to do IHC using the same human antibody. I know the secondary antibody will pick up the antibody that is injected as well as the one I applied as primary antibody in IHC. I hope I said it clearly. So please tell me what kind of blocking I can use to block the injected human antibody? And how? Will normal human serum work? 

Any input will be highly appreciated! 


Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
Histonet mailing list

<< Previous Message | Next Message >>