[Histonet] Double Labeling Same Species

From:"Fleming, Tiffany M"



I do a lot of double-labeling- but my anti-bodies are from different

(i.e; Cfos (rabbit) Oxytocin (mouse) FosB (goat)), with fantastic


I am attempting to stain mice brain tissue for a different P.I with :

Cfos(rabbit) with DAB

GFP (rabbit) with CY3

AVP (rabbit) with Alexa 488


I did a trial run with

Cfos (rabbit) cy3

GFP (rabbit) 488

I am having a terrible time with cross staining/ cross reaction.


The professor has no problem staining with GFP and AVP (both rabbit). I
am using a combination of his protocol with my cfos protocol.

Any suggestions?


Tiffany M. Fleming

Cunningham Lab 225C

UTHSCSA Pharmacology Department

7703 Floyd Curl Dr San Antonio TX 78229-3900

lab (210) 567-4180 (210) 567- 4273

fax 210-567-4300 



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