[Histonet] Double Labeling Same Species
From: | "Fleming, Tiffany M" |
Hello,
I do a lot of double-labeling- but my anti-bodies are from different
species
(i.e; Cfos (rabbit) Oxytocin (mouse) FosB (goat)), with fantastic
results.
I am attempting to stain mice brain tissue for a different P.I with :
Cfos(rabbit) with DAB
GFP (rabbit) with CY3
AVP (rabbit) with Alexa 488
I did a trial run with
Cfos (rabbit) cy3
GFP (rabbit) 488
I am having a terrible time with cross staining/ cross reaction.
The professor has no problem staining with GFP and AVP (both rabbit). I
am using a combination of his protocol with my cfos protocol.
Any suggestions?
Tiffany M. Fleming
Cunningham Lab 225C
UTHSCSA Pharmacology Department
7703 Floyd Curl Dr San Antonio TX 78229-3900
lab (210) 567-4180 (210) 567- 4273
fax 210-567-4300
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