Superfrost slide discussion revisited

From:Gayle Callis

Patsy Ruegg is correct, adding a gelatin based adhesive, i.e. Sta On will
negate the effects of SuperFrost Plus (registered trademark product made by
Erie Scientific) for Fisher, Allegiance, VWR, Richard Allan.   These
companies contract Erie to produce these slides with the company name on
the slides rather than Erie's. 

Plus charge are SILANE coated.  Erie also makes a poly l lysine coated
slide, called PolySine (also trademarked).  

So if one sees Superfrost Plus Charge with registered trademark, this means
Erie Scientific, silane coated.  Merck probably makes a similar slide with
positive charge, as do other companies but they cannot call them Plus Charge. 

Inside slide boxes is an instruction insert that TELLS how to use the
slides, including no waterbath adhesive, AND distilled water IF tap water
is not pure enough in some regions in the country, i.e. extremely hard or
minerals present. It would be in your best interest to read that insert. If
you coat Plus charge (negatively charged slide surface) with some type of
adhesive - commonly gelatin based,  then you are basically doing the same
thing as picking up a section from a pure, distilled waterbath (NO STA-ON
Please!) onto that slide surface.  
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu




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