These dyes are both briefly reviewed, each with 3 or 4
references to recent original papers, in the 10th edition
of Conn's Biological Stains, edited by Richard Horobin
with me as his sidekick. For availability, check out
http://pulish.uwo.ca/~jkiernan/bookfind.htm#CONN

I'm happy to plug this book on the Histonet listserver 
because Richard and I do not receive any royalties from 
sales. The editing was part of our academic work (salaries
paid by universities) and was also supported by the 
Biological Stain Commission. Any profits that might be 
gained from sales of Conn go to the Commission, which has 
been working to improve stains for 78 years. 

If your lab spends $150 on a copy of Conn's you will have a
book full of useful data, and for the price of 0.1 ml of an 
antiserum or 2 grams of osmium tetroxide.

End of plug for the new Conn's. 

The Biological Stain Commission's annual meeting is in
Rochester, NY  on 6th to 7th June. Check out the
web site: 
http://www.biostains.org 
This is the most friendly science/technical meeting
anywhere. As one who hates most meetings I give the
BSC's annual gathering a 100% endorsement!

-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/
______________________________________________________________

Roger Brown wrote:
> 
> Hello HistoNet users,
> 
> I am seeking information on comparative advantages of alizarin
> complexone vs. xylenol orange for fluorochrome labeling of bone in
> rats.  Could anyone comment on which of these two yields brighter
> labeling and recommended doses?  Incidentally, I am hoping to use one
> of these two plus calcein for double fluorochrome labeling.
> 
> Thanks in advance for any assistance.
> 
> --
> 
> ***********************************************************
> Roger F. Brown
> Professor of Biological Sciences        Phone: (573) 341-4860
> E-mail: rbrown@umr.edu          Fax:   (573) 341-4821
> Room 112 Schrenk Hall                   1870 Miner Circle
> University of Missouri-Rolla    Rolla, MO  65409-1120
> 
> ***********************************************************
_________________________________________________________________