undecalcified bone/aquamount mess!
Hi,
I have some immunohistochemically stained sections of undecalcified
joints which have cracked horribly over the last 1-2 years - they
were fine when I did them! the sections are frozen in a block of
wallpaper paste (carboxy methyl cellulose) and I thing it's this
which has dried out and cracked. The trouble is I can't get the
coverslips off (if i remember rightly, I used Gurr aquamount from
BDH) to try to fix the sections by some sort of rehydration. Does
anyone have any idea if a) it is the paste that's cracked, b) how to
get the coverslips off without pulling off the sectoions and c) how
best to rehydrate/re-slip the slides? Any help would be most
appreciated since (as with all specimens) these are precious and I'm
kicking myself for not get images of them when I first did them!
Thanks.
--
Dr Joey Shepherd
Postdoctoral scientist
Academic Unit of Genetics and Informatics
Division of Genomic Medicine
The University of Sheffield Medical School
M Floor, Royal Hallamshire Hospital
Sheffield S10 2JF
UK
Phone 0114 271-3007
Fax 0114-272-1104
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