--Boundary_(ID_1lZqIClfjy2tFG2dMUBxDQ)
Content-type: multipart/alternative;
 boundary="Boundary_(ID_3ESbGoplL/9zqmC5W/YymQ)"


--Boundary_(ID_3ESbGoplL/9zqmC5W/YymQ)
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT

Sigma is no longer making premade Trisma Buffer pH 7.4
We have always used their product in making our solution for the NADH muscle 
stain.  
I've experimented with a Sodium Phosphate diabasic/monobasic solution and the 
results looked the same as a NADH muscle stained with a Trisma buffer 
component.

Can anyone tell me what the difference between the two buffers are and what 
factors I need to consider, if any, in transitioning to making the sodium 
phosphate buffer to replace the Trisma buffer? 

Lastly, does anyone know of a different supplier of premadeTrisma buffers, 
other than Sigma or Fisher (since Fisher gets their tris buffer from Sigma?)

Thanks,
Deb King HT
Sacramento, CA

--Boundary_(ID_3ESbGoplL/9zqmC5W/YymQ)
Content-type: text/html; charset=US-ASCII
Content-transfer-encoding: 7BIT

Sigma is no longer making premade Trisma Buffer pH 7.4

We have always used their product in making our solution for the NADH muscle stain.  

I've experimented with a Sodium Phosphate diabasic/monobasic solution and the results looked the same as a NADH muscle stained with a Trisma buffer component.



Can anyone tell me what the difference between the two buffers are and what factors I need to consider, if any, in transitioning to making the sodium phosphate buffer to replace the Trisma buffer? 



Lastly, does anyone know of a different supplier of premadeTrisma buffers, other than Sigma or Fisher (since Fisher gets their tris buffer from Sigma?)



Thanks,

Deb King HT

Sacramento, CA

--Boundary_(ID_3ESbGoplL/9zqmC5W/YymQ)--

--Boundary_(ID_1lZqIClfjy2tFG2dMUBxDQ)
Content-type: message/rfc822

Return-path: 
Date: Wed, 7 May 2003 22:18:34 EDT
From: WWmn916@aol.com
Subject: Tris or Phosphate buffer?
To: histonet@pathology.swmed.edu
Message-id: <15f.20026f55.2beb187a@aol.com>
MIME-version: 1.0
X-Mailer: 6.0 sub 10577
Content-type: multipart/alternative;
 boundary="Boundary_(ID_7iqcDLFRP7WCJxTbhUzRVQ)"
Full-name: WWmn916


--Boundary_(ID_7iqcDLFRP7WCJxTbhUzRVQ)
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT

Sigma is no longer making premade Trisma Buffer pH 7.4
We have always used their product in making our solution for the NADH muscle 
stain.  
I've experimented with a Sodium Phosphate diabasic/monobasic solution and the 
results looked the same as a NADH muscle stained with a Trisma buffer 
component.

Can anyone tell me what the difference between the two buffers are and what 
factors I need to consider, if any, in transitioning to making the sodium 
phosphate buffer to replace the Trisma buffer?  

Lastly, does anyone know of a different supplier of premadeTrisma buffers, 
other than Sigma or Fisher (since Fisher gets their tris buffer from Sigma?)

Thanks,
Deb King HT
Sacramento, CA

--Boundary_(ID_7iqcDLFRP7WCJxTbhUzRVQ)
Content-type: text/html; charset=US-ASCII
Content-transfer-encoding: 7BIT

Sigma is no longer making premade Trisma Buffer pH 7.4

We have always used their product in making our solution for the NADH muscle stain.  

I've experimented with a Sodium Phosphate diabasic/monobasic solution and the results looked the same as a NADH muscle stained with a Trisma buffer component.



Can anyone tell me what the difference between the two buffers are and what factors I need to consider, if any, in transitioning to making the sodium phosphate buffer to replace the Trisma buffer?  



Lastly, does anyone know of a different supplier of premadeTrisma buffers, other than Sigma or Fisher (since Fisher gets their tris buffer from Sigma?)



Thanks,

Deb King HT

Sacramento, CA

--Boundary_(ID_7iqcDLFRP7WCJxTbhUzRVQ)--

--Boundary_(ID_1lZqIClfjy2tFG2dMUBxDQ)--