RE: Tris or Phosphate buffer?

From:Tony Henwood

Try Shandon

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: []
Sent: Monday, 12 May 2003 13:26
Subject: Tris or Phosphate buffer?

Sigma is no longer making premade Trisma Buffer pH 7.4
We have always used their product in making our solution for the NADH muscle stain.  
I've experimented with a Sodium Phosphate diabasic/monobasic solution and the results looked the same as a NADH muscle stained with a Trisma buffer component.

Can anyone tell me what the difference between the two buffers are and what factors I need to consider, if any, in transitioning to making the sodium phosphate buffer to replace the Trisma buffer?

Lastly, does anyone know of a different supplier of premadeTrisma buffers, other than Sigma or Fisher (since Fisher gets their tris buffer from Sigma?)

Deb King HT
Sacramento, CA

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