RE: Source for AEC chromogen

From:"C.M. van der Loos"

Dear Teri:
Just try the following protocol:

AEC stock solution: 1000 mg AEC (Sigma A5754) in 100 ml N,N-
dimethylformamide. Store in dark at 4C.

2.5 ml AEC stock solution + 45.5 ml Na-acetate buffer (pH 4.9, 50 mM)
Filter directly!
Just before use add 20 ul peroxide (30%).
Stain for 5-10 min at RT in a coplin jar.

This protocol yields stronger staining intensities than most commercial 
available AEC based kit systems.
Happy staining!

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands

Teri Johnson wrote:
>Date: 29 Apr 2003 16:45:39 -0500
>From: "Johnson, Teri" 
>Subject: Source for AEC chromogen
>I just sent in an order for AEC+ and found the cost has more than 
>(nearly tripled) since I bought it last. I'm looking for alternatives 
>that are
>somewhat less costly without sacrificing quality (very important).  
>What are
>you all using out there for AEC?  I prefer DAB, but the researcher I'm
>staining for likes the red product.  I have Vector Nova Red, but since 
>one has to make up 5 ml at a time and I don't use that much, I hate to 
>waste the leftovers.
>Thanks for your help!
>Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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