RE: Source for AEC chromogen
Just try the following protocol:
AEC stock solution: 1000 mg AEC (Sigma A5754) in 100 ml N,N-
dimethylformamide. Store in dark at 4C.
2.5 ml AEC stock solution + 45.5 ml Na-acetate buffer (pH 4.9, 50 mM)
Just before use add 20 ul peroxide (30%).
Stain for 5-10 min at RT in a coplin jar.
This protocol yields stronger staining intensities than most commercial
available AEC based kit systems.
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands
Teri Johnson wrote:
>Date: 29 Apr 2003 16:45:39 -0500
>From: "Johnson, Teri"
>Subject: Source for AEC chromogen
>I just sent in an order for AEC+ and found the cost has more than
>(nearly tripled) since I bought it last. I'm looking for alternatives
>somewhat less costly without sacrificing quality (very important).
>you all using out there for AEC? I prefer DAB, but the researcher I'm
>staining for likes the red product. I have Vector Nova Red, but since
>one has to make up 5 ml at a time and I don't use that much, I hate to
>waste the leftovers.
>Thanks for your help!
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
<< Previous Message | Next Message >>