RE: ALkaline phosphatase Ab for confocal

From:"C.M. van der Loos"

Hi Phil,
Vector Red as chromogen for AP activity yields a reaction product that 
is brighly red fluorescent when emitted with a rhodamin filter pack. 
The fluorescence signal tends to be very low fading (1000x less than 
FITC!) and is also useful for confocal techniques.

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands

Phil Bergin wrote:
>Date: 29 Apr 2003 07:34:40 -0500
>From: Phil Bergin 
>Subject: ALkaline phosphatase Ab for confocal
>I have a colleague interested in staining cytospun cells for Alkaline
>phosphatase to localize it in her cells.  Then to analyze it using
>confocal microscopy.  Does anyone have any advice on how to fix the
>cells and a suitable Ab or kit?  She has tried the ELF 97 kit from
>molecular probes with no success.
>Many thanks in advance for the help.

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