CD138 and EMA on Ventana
We are having a puzzling result form some of our CD138 slides which we are
staining on a Ventana immunostainer. In some tissues (but not all), we are
getting strong nuclear staining. We do not get this result when we stain by
hand. The neg. control is neg. Today we had a bone marrow plug biopsey that
was showing this effect, we have also seen it in tonsil and other lymphoid
tissue. Blocking does not solve the problem. We need to tinker more with the
titre and length of staining (Ab) time some more. Has anyone else
encountered this with CD138?
Another problem we had seems specific to one case so far, but I am sure we
will see it again. An EMA we did by hand showed good staining of the tumour
cells, but when done on the Ventana stainer, no matter what we do to it in
terms of pretreatment, the tumour cells are neg. We have tinkered with
titre, timing, protease, retrieval at various lengths, and so on. Have
others had this problem with EMA on Ventana?
Thanks for you assistance.
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