frozen sections
I wonder if anyone can help me on this particular subject. I
have a PhD student from the College of Agriculture and Marine Sciences who is
looking at the proportion of lipid in gonads during the breeding season. The
problem is that he fixed his samples in NBF and has now decided he wants to do
frozen sections for fat stains. Ordinarily I would have said no problem, as I
have carried many a frozen section on tissues previously fixed in NBF(after
suitable washing in water). In this case however, at cryotomy the tissue just
crumbles. Has anyone else had this problem? Can anyone make any sugestions? I
have thought of gelatine embedding but as he has 110 gonads (no jokes
please) to sample, I am looking for an easier alternative to suggest to
him.
Evelyn Kaplan
Dept of Pathology,
Sultan Qaboos University,
Oman
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