Re: Citrate buffer
Re: Citrate buffer
I use a Black & Decker Model
HS2000 Vegetable Steamer which we ordered off the Black & Decker
web-site ($40-50 US). It even has a chart right on it with
different times, depending on what is being steamed, although it does
not have anything about slices of tissues on mounted on slides, so the
chart isn't much help. I steam 20 minutes (after it has
preheated) in Coplin jars of pre-heated (in the microwave)
buffer.
Susan Bell
Research Assistant
Targeted Genetics Corporation
1100 Olive Way Suite 100
Seattle WA 98101
Phone: 206-521-4830
Fax: 206-223-0288
bells@targen.com
At 12:14 PM -0400 5/9/02, Vinnie Della Speranza wrote:
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>>> Brenda D Begg <blproverbs@rediffmail.com> 02/05/02
09:30AM >>>
Hi Guys,
I would like to use heated citrate buffer as antigen retrival agent.
Everyone keeps talking about a microwave or steamer or pressure cooker
none of which I have (I don't live in the states so these things are
expensive). Does anyone know of a method involving a simple heater or
bunsen burner.
Thanks
Brenda B.
--
Susan Bell
Research Assistant
Targeted Genetics Corporation
1100 Olive Way Suite 100
Seattle WA 98101
Phone: 206-521-4830
Fax: 206-223-0288
bells@targen.com
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