Re: Citrate buffer

From:"ander093@tc.umn.edu"

Responding to the message of 
from Susan Bell :

We use a Sunbeam vegetable steamer purchased from Target (KMart, Walmart, etc.) 
for about $30. Steam from 15-30 minutes depending on antibody, using capillary 
gap slides.  Works great!!

LuAnn Anderson; HT(ASCP)
Assistant Scientist
Neuropathology Lab
University of Minnesota
> 
> I use a Black & Decker Model HS2000 Vegetable Steamer which we 
> ordered off the Black & Decker web-site ($40-50 US).  It even has a 
> chart right on it with different times, depending on what is being 
> steamed, although it does not have anything about slices of tissues 
> on mounted on slides, so the chart isn't much help.  I steam 20 
> minutes (after it has preheated) in Coplin jars of pre-heated (in the 
> microwave) buffer.
> 
> Susan Bell
> Research Assistant
> Targeted Genetics Corporation
> 1100 Olive Way  Suite 100
> Seattle WA  98101
> Phone:  206-521-4830
> Fax:  206-223-0288
> bells@targen.com
> 
> At 12:14 PM -0400 5/9/02, Vinnie Della Speranza wrote:
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> >
> >
> >
> >>>>  Brenda D Begg  02/05/02 09:30AM >>>
> >
> >Hi Guys,
> >I would like to use heated citrate buffer as antigen retrival agent. 
> >Everyone keeps talking about a microwave or steamer or pressure 
> >cooker none of which I have (I don't live in the states so these 
> >things are expensive). Does anyone know of a method involving a 
> >simple heater or bunsen burner.
> >Thanks
> >Brenda B.
> 
> 
> -- 
> 
> 
> Susan Bell
> Research Assistant
> Targeted Genetics Corporation
> 1100 Olive Way  Suite 100
> Seattle WA  98101
> Phone:  206-521-4830
> Fax:  206-223-0288
> bells@targen.com
> 
> --Boundary_(ID_kL56Cv9cPhUh6vDGehgVQA)
> Content-type: text/html; charset=us-ascii
> Content-transfer-encoding: 7BIT
> 
> 
> Re: Citrate buffer
> 
I use a Black & Decker Model > HS2000 Vegetable Steamer which we ordered off the Black & Decker > web-site ($40-50 US).  It even has a chart right on it with > different times, depending on what is being steamed, although it does > not have anything about slices of tissues on mounted on slides, so the > chart isn't much help.  I steam 20 minutes (after it has > preheated) in Coplin jars of pre-heated (in the microwave) > buffer.
>

>
Susan Bell
> Research Assistant
> Targeted Genetics Corporation
> 1100 Olive Way  Suite 100
> Seattle WA  98101
> Phone:  206-521-4830
> Fax:  206-223-0288
>
bells@targen.com color="#000000">        >         >         >
>

>
At 12:14 PM -0400 5/9/02, Vinnie Della Speranza wrote:
>
Content-type: text/html; > charset=ISO-8859-1
> Content-description: HTML
> Content-Transfer-Encoding: 7BIT
>
>

>
> >>> Brenda D Begg <blproverbs@rediffmail.com> 02/05/02 > 09:30AM >>>
>
> Hi Guys,
> I would like to use heated citrate buffer as antigen retrival agent. > Everyone keeps talking about a microwave or steamer or pressure cooker > none of which I have (I don't live in the states so these things are > expensive). Does anyone know of a method involving a simple heater or > bunsen burner.
>
Thanks
>
Brenda B.
>

>

>
-- 
> 
>

>
>
>
Susan Bell
> Research Assistant
> Targeted Genetics Corporation
> 1100 Olive Way  Suite 100
> Seattle WA  98101
> Phone:  206-521-4830
> Fax:  206-223-0288
>
bells@targen.com
> > > > --Boundary_(ID_kL56Cv9cPhUh6vDGehgVQA)-- > > .

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