02/05/02 09:30AM >>>
> >
> >Hi Guys,
> >I would like to use heated citrate buffer as antigen retrival agent.
> >Everyone keeps talking about a microwave or steamer or pressure
> >cooker none of which I have (I don't live in the states so these
> >things are expensive). Does anyone know of a method involving a
> >simple heater or bunsen burner.
> >Thanks
> >Brenda B.
>
>
> --
>
>
> Susan Bell
> Research Assistant
> Targeted Genetics Corporation
> 1100 Olive Way Suite 100
> Seattle WA 98101
> Phone: 206-521-4830
> Fax: 206-223-0288
> bells@targen.com
>
> --Boundary_(ID_kL56Cv9cPhUh6vDGehgVQA)
> Content-type: text/html; charset=us-ascii
> Content-transfer-encoding: 7BIT
>
>
> Re: Citrate buffer
> I use a Black & Decker Model
> HS2000 Vegetable Steamer which we ordered off the Black & Decker
> web-site ($40-50 US). It even has a chart right on it with
> different times, depending on what is being steamed, although it does
> not have anything about slices of tissues on mounted on slides, so the
> chart isn't much help. I steam 20 minutes (after it has
> preheated) in Coplin jars of pre-heated (in the microwave)
> buffer.
>
> Susan Bell
> Research Assistant
> Targeted Genetics Corporation
> 1100 Olive Way Suite 100
> Seattle WA 98101
> Phone: 206-521-4830
> Fax: 206-223-0288
> bells@targen.com color="#000000">
>
>
>
>
> At 12:14 PM -0400 5/9/02, Vinnie Della Speranza wrote:
> Content-type: text/html;
> charset=ISO-8859-1
> Content-description: HTML
> Content-Transfer-Encoding: 7BIT
>
>
>
> >>> Brenda D Begg <blproverbs@rediffmail.com> 02/05/02
> 09:30AM >>>
>
> Hi Guys,
> I would like to use heated citrate buffer as antigen retrival agent.
> Everyone keeps talking about a microwave or steamer or pressure cooker
> none of which I have (I don't live in the states so these things are
> expensive). Does anyone know of a method involving a simple heater or
> bunsen burner.
> Thanks
> Brenda B.
>
>
> --
>
>
>
>
> Susan Bell
> Research Assistant
> Targeted Genetics Corporation
> 1100 Olive Way Suite 100
> Seattle WA 98101
> Phone: 206-521-4830
> Fax: 206-223-0288
> bells@targen.com
>
>
>
> --Boundary_(ID_kL56Cv9cPhUh6vDGehgVQA)--
>
> .