Fwd: muscle biopsies

From:"Dr. Ian Montgomery"

        There is only one sure method of freezing muscle biopsies and that is iso-pentane cooled using liquid nitrogen, anything else can and usually does introduce ice crystal artifact. Once frozen the specimens can be shipped safely using dry ice.

Date: Thu, 16 May 2002 14:45:20 -0700
From: "Histology, Metro Lab" <histo@qcmetro.com>
Subject: muscle biopsies
To: histonet posting address <histonet@pathology.swmed.edu>

We send our muscle biopsies to a referral lab.  Their protocol is to freeze the muscle in a mixture of acetone and dry ice and then ship the specimen in dry ice.  We have a problem of freeze artifact, that is in the report, that the lab send ours.   Does anyone do a process, like this and if so, do you have any suggestions, on how to eliminate the freeze artifact?
Deb Croegaert
Metro Labs
Davenport, Iowa

Dr. Ian Montgomery,
Histotechnology Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
e-mail: ian.montgomery@bio.gla.ac.uk

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