From:"Van Eyck, Deb"

  Here's an immuno question for everyone doing long term storage of concentrated antibodies???  What method have you found to work best?  We are doing more concentrates, and I have small cryo-vials and a -70 freezer. Most directions state freeze in volume needed at later date.  Do you just take off that dilution amount of concentrated antibody needed to make your final and freeze that (seems like almost too tiny an amount) then thaw and rinse out the vial with the correct amount of diluent---does this work or is it less than scientific?? Or do you make up your antibody in diluent and freeze the whole shabang???  Please share your time proven methods?   Also any input on saving already cut secti ons of controls in the freezer?  Which ones do you find this helpful for?  Thanks very much! 

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