It is not recommended to freeze diluted antibody so the best way is to aliquot the concentrated antibody and freeze.

Dorit

-----Original Message-----
From:	Van Eyck, Deb [SMTP:deb.vaneyck@phci.org]
Sent:	Sat 04 May 2002 1:50
To:	'Histonet@pathology.swmed.edu'
Subject:	

	
  Here's an immuno question for everyone doing long term storage of
concentrated antibodies???  What method have you found to work best?  We are
doing more concentrates, and I have small cryo-vials and a -70 freezer. Most
directions state freeze in volume needed at later date.  Do you just take
off that dilution amount of concentrated antibody needed to make your final
and freeze that (seems like almost too tiny an amount) then thaw and rinse
out the vial with the correct amount of diluent---does this work or is it
less than scientific?? Or do you make up your antibody in diluent and freeze
the whole shabang???  Please share your time proven methods?   Also any
input on saving already cut sections of controls in the freezer?  Which ones
do you find this helpful for?  Thanks very much!  
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