Hi,
    Toss in a slice of bread and it's poached egg with milk toast ... that's
it ... I'm going on break I'm hungry :-)
Amos Brooks
----- Original Message -----
From: "Patti Loykasek" <ploykasek@phenopath.com>
To: "histonet" <histonet@pathology.swmed.edu>
Sent: Monday, May 07, 2001 11:50 AM
Subject: Re: Egg as a biotin blocker!


> We use egg substitute that you buy at the grocery. This product is 97-98%
> egg white. We use 75ml of the egg in 200ml of distilled water. Leave
slides
> in for 15 minutes. Wash in 2 changes of distilled water. Put slides in
skim
> milk for 15 minutes. Wash in 2 changes of distilled water. Any buffers
will
> precipitate out the eggs, so be sure and use water. Dr. Rodney Miller
> introduced this method of biotin blocking at a workshop I attended. The
eggs
> contain avidin, and the milk is a source of biotin. Hope this helps.
>
> Patti Loykasek
>
>