RE: IHC storage alcohol

From:"Morken, Tim" <>

We routinely store our tissues in 70 percent ethanol to preserve

Tim Morken, BA, EMT(MSA), HTL(ASCP)
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention
1600 Clifton Road
Atlanta, GA 30333

PH: 404-639-3964
FAX: 404-639-3043


-----Original Message-----
From: Jan Shivers []
Sent: Thursday, May 03, 2001 4:02 PM
To: HistoNet Server
Subject: IHC storage alcohol

Usually the tissue samples I receive have been optimally fixed (time-wise)
in formalin and promptly embedded into paraffin.  Today I received a call
from a researcher out of state who wants to send me some samples, but cannot
get his fixed tissue processed into paraffin within a 24-hour period after
start of fixation.  He wants to know what storage alcohol he should switch
the samples into until embedding, and I became brain-dead.  Can someone help
me out here PDQ?  Is it 70% ethanol or some other percentage?  Is there a
better storage medium than ethanol?  I have received some cases in PBS
(post-formalin-fixation), but those staining results were less than optimal.

Any help would be greatly appreciated.

Jan Shivers
Univ. of Minn. Vet. Diag. Lab

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