Manganese staining

From:Patsy.Ruegg@UCHSC.edu

 

Mn is usually  measured indirectly by differing combinations of urine,
blood serum,  hair samples, an  MRI, or monitoring the activity of
manganese-activated enzymes and metalloenzymes etc.  None of these
methods
are perfect biomarkers.  John says if one is a good chemist, you might
be
able to made up a stain of permanganate like a Potassium permangate when
applied to a slide may glow purple( this is a guess).  I  also looked
for
stains in literature but did not find any, when I was looking at
toxicity.
Manganese often appears not to stay in tissue, rather leaves a trail of
damage which progresses after the withdrawal of exposure.

On Thu, 3 May 2001 Patsy.Ruegg@UCHSC.edu wrote:

> Hi Kathy,
> Was wondering if John could help with this inquiry?
> Patsy
>
> -----Original Message-----
> From:	Histo-Scientific Research Laboratories
[mailto:histosci@shentel.net]
> <mailto:[mailto:histosci@shentel.net]>
> Sent:	Thursday, May 03, 2001 6:08 AM
> To:	Linda McGraf
> Subject:	Manganese staining
>
> Dear Histonetters,
>
> Has anyone ever heard of staining for localization of manganese in
animal
> tissue?  We have looked through our staining books and have come up
with
> nothing.  If anyone has ever heard of such a procedure, please share
any
> info you may have.
> 				Thank you,
>
> 				Beth Poole
> 				HSRL
> 				beth@hsrl.org <mailto:beth@hsrl.org>
> 				(540)459-8211
> 				fax: (540)459-8217
> 				www.hsrl.org <http://www.hsrl.org>
> 				137 South Main Street
> 				Woodstock, VA  22664
>
>



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