carrie,
the poly cd 31 i used from RD is cat. # RDI-MCD31CabG goat anti-CD31
(PECAM-1) but we do not like it very much.  we prefer a more specific
antibody for endothelial cells from Santa Cruz listed as Endoglin TGFbRIII
(C20) cat.# sc-6199.
patsy ruegg

-----Original Message-----
From: Cartwright, Carrie [mailto:carrie.cartwright@Agouron.COM]
Sent: Monday, May 07, 2001 1:02 PM
To: 'Patsy.Ruegg@UCHSC.edu'; jennifer.philopena@canji.com;
histonet@pathology.swmed.edu
Subject: RE: CD31/F8



Hi Patsy! 
        I was wondering which poly CD31 you used from Research Diagnostics.
I tried one before and never got any results.  Do you use it on paraffin or
frozen?  I work with whole mount retinas, so that may be the difference.  I
did find a good monoclonal from Pharmingen that has worked for me.  Finding
a poly that works is preferable though because I do a lot of dual staining.

Carrie 

-----Original Message----- 
From: Patsy.Ruegg@UCHSC.edu [ mailto:Patsy.Ruegg@UCHSC.edu
<mailto:Patsy.Ruegg@UCHSC.edu> ] 
Sent: Monday, April 30, 2001 8:32 AM 
To: jennifer.philopena@canji.com; histonet@pathology.swmed.edu 
Subject: CD31/F8 


For both of these antibodies in my experience the polyclonal antibodies work

better than the monoclonal, I use DAKO's poly F8 and Research Diagnostics 
poly CD31. 
Patsy Ruegg 

                -----Original Message----- 
                From:   Philopena, Jennifer 
[ mailto:jennifer.philopena@canji.com <mailto:jennifer.philopena@canji.com>
] 
                Sent:   Thursday, April 26, 2001 5:53 PM 
                To:     'Histonet' (E-mail) 
                Subject:        

                Hi there. 
                Does anyone have a protocol that they would be willing to 
share to detect 
                PECAM (CD31) or Von Willebrand factor in FFPE tissue?  I 
have it worked out 
                nicely in frozens. 
                Thanks Thanks Thanks 
                Jen 

        
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