Katie Bennett of Lovelace Resp. res. Inst. Faxed me her procedure for brduin
GMA (Immunobed).  Don't have her email address handy but here is her phone
number 505-845-1118  I think she is at Michigan State.
Patsy Ruegg

		-----Original Message-----
		From:	Deb Boswell Lane [mailto:dbl@aretha.jax.org]
		Sent:	Wednesday, May 02, 2001 7:46 AM
		To:	Histonet@pathology.swmed.edu
		Subject:	BrdU in Unicryl??

		I'm relaying a question from a grad student:
		Limulus (horseshoe crab) was embedded in Unicryl and initial
attempts at
		immunohistochemistry for BrdU haven't worked.  Has anyone
done BrdU immuno
		work for light microscopy in Unicryl or similar media?  And
does anyone
		have any suggestions for keeping 10 micron thick paraffin
sections of
		Limulus (horseshoe crab) tissue on slides through IHC
procedures?  Please
		make suggestions!  This if for a dissertation?

		Thanks in advance!

		Deb
		Deb Boswell Lane
		Professional Biomedical Technologist
		Biological Imaging
		The Jackson Laboratory
		600 Main Street
		Bar Harbor, Maine 04609
		(207) 288-6193
		dbl@jax.org