RE: BrdU in Unicryl??

A good way to keep sections on the slides if you are not doing collagen
markers (the glue interfers with staining) is to precoat the slides (reg.
Slides) with a solution of 5% elmers type glue in water, dip the slides in
this then let them air dry.  Pick up your sections onto the glue coated
slides and dry them as usual, they stick really well, I use this for rabbit
bones, but beware that glue is collagen based and will cause problems with
some IHC markers.
Patsy Ruegg

		-----Original Message-----
		From:	Deb Boswell Lane []
		Sent:	Wednesday, May 02, 2001 7:46 AM
		Subject:	BrdU in Unicryl??

		I'm relaying a question from a grad student:
		Limulus (horseshoe crab) was embedded in Unicryl and initial
attempts at
		immunohistochemistry for BrdU haven't worked.  Has anyone
done BrdU immuno
		work for light microscopy in Unicryl or similar media?  And
does anyone
		have any suggestions for keeping 10 micron thick paraffin
sections of
		Limulus (horseshoe crab) tissue on slides through IHC
procedures?  Please
		make suggestions!  This if for a dissertation?

		Thanks in advance!

		Deb Boswell Lane
		Professional Biomedical Technologist
		Biological Imaging
		The Jackson Laboratory
		600 Main Street
		Bar Harbor, Maine 04609
		(207) 288-6193

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