RE: BrdU in Unicryl??
A good way to keep sections on the slides if you are not doing collagen
markers (the glue interfers with staining) is to precoat the slides (reg.
Slides) with a solution of 5% elmers type glue in water, dip the slides in
this then let them air dry. Pick up your sections onto the glue coated
slides and dry them as usual, they stick really well, I use this for rabbit
bones, but beware that glue is collagen based and will cause problems with
some IHC markers.
From: Deb Boswell Lane [mailto:email@example.com]
Sent: Wednesday, May 02, 2001 7:46 AM
Subject: BrdU in Unicryl??
I'm relaying a question from a grad student:
Limulus (horseshoe crab) was embedded in Unicryl and initial
immunohistochemistry for BrdU haven't worked. Has anyone
done BrdU immuno
work for light microscopy in Unicryl or similar media? And
have any suggestions for keeping 10 micron thick paraffin
Limulus (horseshoe crab) tissue on slides through IHC
make suggestions! This if for a dissertation?
Thanks in advance!
Deb Boswell Lane
Professional Biomedical Technologist
The Jackson Laboratory
600 Main Street
Bar Harbor, Maine 04609
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