IHC storage alcohol
|From:||Jan Shivers <email@example.com>|
Usually the tissue samples I receive have been optimally fixed (time-wise)
in formalin and promptly embedded into paraffin. Today I received a call
from a researcher out of state who wants to send me some samples, but cannot
get his fixed tissue processed into paraffin within a 24-hour period after
start of fixation. He wants to know what storage alcohol he should switch
the samples into until embedding, and I became brain-dead. Can someone help
me out here PDQ? Is it 70% ethanol or some other percentage? Is there a
better storage medium than ethanol? I have received some cases in PBS
(post-formalin-fixation), but those staining results were less than optimal.
Any help would be greatly appreciated.
Univ. of Minn. Vet. Diag. Lab
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