RE: E-Cadherin

From:"Baldridge, Lee Ann" <lhadley@iupui.edu>

Theresa,
Just worked on that one today.  I used Cell Marque's prediluted E-Cadherin
with Dako's Target Retrievel and LSAB2.  Primary antibody incubation at
60min. with the link and label at 20min each.  DAB 5min.  Very pretty!
Good Luck
Lee Ann Baldridge
IU Hosp.
Indpls. IN
317-274-5790
317-278-4820 fax

-----Original Message-----
From: THERESA ROHR [mailto:trohratnyackhospital@hotmail.com]
Sent: Thursday, May 03, 2001 2:01 PM
To: histonet@pathology.swmed.edu
Subject: E-Cadherin


Hello Histonetters,

My Pathologist would like me to get E-Cadherin up and running. Since we are 
having budget problems, I called around trying to get a free sample. Cell 
Marque sent me a sample, a .05ml tube. When I called their Tech Service to 
get a starting titer, they recommended a 1 to 5 dilution. That minute amount

not only negates my DAKO Autostainer but would be outrageously expensive for

me.  I am using the Envision Plus detection system. Are any of you using 
this antibody? What company is your supplier? What is the dilution? Any 
suggestions are welcome.

Thanks,
Theresa Rohr
Section Head, Histology
Nyack Hospital, NY
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