From:Shawn Griffin <SGriffin@ameripath.com>

Hey Richard

One of our doctors went to a meeting and they talked about RCC.  When she
came back she had us order it.  We have only used it a few times and she
seems to be pleased with the results. Maybe you have found something out.


-----Original Message-----
From: Richard Cartun [mailto:Rcartun@harthosp.org] 
Sent: Tuesday, May 01, 2001 10:40 AM
To: SGriffin@ameripath.com
Subject: Re: FW: RCC

Hi Shawn:

Do you find this antibody useful?  It appears to lack sensitivity
(identifying metastatic renal cell CA) from the work that I have done.

R. Cartun

>>> Shawn Griffin <SGriffin@ameripath.com> 04/27/01 11:49AM >>>

-----Original Message-----
From: Shawn Griffin 
Sent: Friday, April 27, 2001 4:06 AM
To: 'Patti Loykasek'
Subject: RE: RCC


We use RCC, clone 66.4c2 on paraffin tissue from NovoCastra.  We pretreat
the slides for 20 min using the steamer method.  We use everything from
Dako. The kit we are using is LSAB2HRP.  The dilution is 1:100 for 30
minutes.  I hope this will help you some.

Shawn Griffin
Ameripath, Dallas

-----Original Message-----
From: Patti Loykasek [mailto:ploykasek@phenopath.com] 
Sent: Thursday, April 26, 2001 5:11 PM
To: histonet
Subject: RCC

Can anyone recommend a RCC clone that works on FFPE tissue? Pretreatments?
We have tried several clones and pretreatments with inconsistent results.
Thanks for the help.

Patti Loykasek

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