<br>
<br>
<br><font size=2 face="sans-serif"> Histonetters,</font>
<br>
<br><font size=2 face="sans-serif"> I have a quick question concerning slides coated with Elmer's Glue. I have never used this technique before so I searched the Histonet Archives and found reference for preparing the slides. My question is: will the Glue interfer with H&E and T-Blue staining? I know that it wasn't recommended for IHC. I am currently working on a project with rat ankle joints in which the area of interest includes 3 different tissue types. One small piece of cartilage does not stick well to the slide (positively charged) after drying and subsequently pops off/folds over during staining. It was suggested that I try drying the slides laying flat on a hotplate overnight and also that I use Elmer's Glue coated slides. Does anyone else have any further suggestions. Thank you very much for your insight!</font>
<br>
<br><font size=2 face="sans-serif">Jennifer Hoover</font>
<br><font size=2 face="sans-serif">Eli Lilly and Company</font>