Re: biotin blocking and non-immune sera for negative control
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| From: | Connie McManus <conmac@cc.usu.edu> |
| To: | Amos Brooks <atbrooks@snet.net>, Sonia Greco <soniaG@qimr.edu.au> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
At 08:49 PM 05/20/2000 -0400, Amos Brooks wrote:
>Hi,
> The blocking is put on just before the primary antibody then it is
drained or blown off (not rinsed).
REALLLY??!! I was told to use it before H2O2 and rinse vigorously in
TRIS-HCl with 0.5% Tween. We have had very good results with what I've
been doing.
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA
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