Re: biotin blocking and non-immune sera for negative control

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From:Connie McManus <>
To:Amos Brooks <>, Sonia Greco <>
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At 08:49 PM 05/20/2000 -0400, Amos Brooks wrote:
>    The blocking is put on just before the primary antibody then it is
drained or blown off (not rinsed). 

REALLLY??!!  I was told to use it before H2O2 and rinse vigorously in
TRIS-HCl with 0.5% Tween.  We have had very good results with what I've
been doing.
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT

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