nuclear stain
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From: | Heike Grabsch <h.grabsch@uni-koeln.de> |
To: | histonet@pathology.swmed.edu |
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Content-Type: | text/plain; charset=us-ascii |
Hi Histonetters,
I am sure there is somebody out there who is able to help us! WE do
double immunhistochemistry labeling, one chromogen is DAB+copper sulfate
(nively black) for detection of a nuclear antigen, the other is AEC-one
step solution from Biogenex which gives you a real wonderful red fro a
cytoplasmic antigen. However, I would like to counterstain all other
nuclei. I thought about methyl green, however either it is alcohol-based
like in Romeis (germany histotechnology book) or need to be at least
dehydrated via n-butanol and xylol linke in John Kiernan's book. But
alcohol will kill my AEC-staining! Any other green nuclear stain or may
be yellow which is non-alcohol-based and does not need to be dehydrated?
Thanks for your help.
Heike Grabsch
Dept of Pathology
University of Duesseldorf
Germany
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