nuclear stain

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From:Heike Grabsch <>
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Hi Histonetters,

I am sure there is somebody out there who is able to help us! WE do 
double immunhistochemistry labeling, one chromogen is DAB+copper sulfate 
(nively black) for detection of a nuclear antigen, the other is AEC-one 
step solution from Biogenex which gives you a real wonderful red fro a 
cytoplasmic antigen. However, I would like to counterstain all other 
nuclei. I thought about methyl green, however either it is alcohol-based 
like in Romeis (germany histotechnology book) or need to be at least 
dehydrated via n-butanol and xylol linke in John Kiernan's book. But 
alcohol will kill my AEC-staining! Any other green nuclear stain or may 
be yellow which is non-alcohol-based and does not need to be dehydrated?

Thanks for your help.

Heike Grabsch
Dept of Pathology
University of Duesseldorf

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