Re: Re - Microwave tissue processing
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From: | Valleygal@aol.com |
To: | atbrooks@snet.net, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
>Our lab tried this a few years ago with very poor results. Proceed with
care. Sometimes the longer routine methods are just better. Chemistry is not a
science for the impatient.
Amos Brooks<
Microwave tissue processing DOES produce good - even excellent - results but
care should be taken before you embark on this type of tissue processing. You
are right in your statement that this is not a science for the impatient -
you have to do your homework. If you are using a "kitchen" or "household"
microwave oven you must remember to properly calibrate it and to determine
the processing times that will process not "cook" your tissue. Aside from
venting of vapors from the chemicals being used, accurate time and
temperature control and other important features, laboratory microwave ovens
are designed to make the calibration steps and tissue processing easy. There
are now several on the market.
In addition, there are two books which have been mentioned before on histonet
- The Microwave Tool Book by Login and Dvorak and The Microwave Cookbook by
Kok and Boon that will guide you through the basics of calibration of
microwaves and microwaving tissue.
This isn't a plug...but I am doing a microwave tissue processing workshop
with Donna Willis and Jan Minshew at NSH this year where you can have a hands
on microwaving experience and receive procedures that have been proven to
work and also have a chance to see the results with your own eyeballs!
Andi Grantham
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