Re: Caspase

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From:Debbie Pepperal <>
To:Luis Chiriboga <>
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Hmmmm.....Caspases, I am so glad someone else has a similar problem
regarding this antibody. I have used the one from Dako ( polclonal) on
human lymphoid tissue; it does work, but not perfectly. some cells stain
beautifully and then there are those that are very "muddy", a bit difficult
to interpret. I donot know whether to call them positive or ignore them.
Not a crisp stain. Dako Australia is trying to help optimise it , as I have
tried just about everything possible. I have in the interim , a sample from
Novocastra...which is the new monoclonal. I will inform you of my progress
if you are interested. Hope you get some joy from the Transduction lab
antibody. Please let me know if it is any good!

Thank you

Zenobia Haffajee
HAPS, Newcastle, NSW

At 02:48 PM 24/05/00 -0400, you wrote:
>Hi Netters;
> I am looking for information and recommendations for  sources of Active
>& Non-active Caspase 3 antibodies.  It will be used in human liver
>tissues predominately, but may also be applied to animal species as
>well.  I have used the polyclonal Caspase 3 from PharMingen and it is
>somewhat inconsistent.  I also have the monoclonal Caspase 3 from
>Transduction Labs but I have not worked it up yet.  Anybody have the
>conditions for this marker?
>Thanks in advance
>Luis Chiriboga Ph.D HT(ASCP)QIHC
>Department of Pathology 4W27
>Bellevue Hospital
>NYU School of Medicine
>First Avenue & 27th Street
>New York, NY 10016

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