RE: Artifact: poor fixation or inadequate slide drying
<< Previous Message | Next Message >>
From: | "Weems, Joyce" <JWEEMS@sjha.org> |
To: | 'Adrienne Vair' <a_vair@hotmail.com>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain |
If you coverslip from a xylene substitute - you might try using xylene
again. That stopped the identical complaints of our pathologists. Good luck!
j
> -----Original Message-----
> From: Adrienne Vair [SMTP:a_vair@hotmail.com]
> Sent: Monday, May 29, 2000 1:57 AM
> To: histonet@pathology.swmed.edu
> Subject: Artifact: poor fixation or inadequate slide drying
>
> Our pathologist's are concerned with the poor nuclear morphology they have
>
> been routinely seeing for many years; foamy appearance, muddy nuclear
> staining (H&E). I suggested that we look at extending the time in 10%
> formalin as up until then, surgical specimens were received throughout the
>
> day from the O.R. in 10% Neutral Buffered Formalin (NBF) and most were
> being
> processed the same day with the exception of bowel, uterus, and breast
> tissue which were being opened and fixed 24 hours. We extented the
> fixation
> time to a minimum of 24 hours for all surgical specimens including
> colposcopy, endoscopy and fine needle biopsies but to no avail (and the
> increase in turn around time concerned the physicians). The morphology
> remained poor. I looked at temperatures of all reagents during processing
> and embedding, and checked the pH of the 10% formalin being made up and
> used
> routinely. I stopped using the microwave oven to dry slides as I could not
>
> find any literature to confirm that microwaving a rack of 20 slides for 7
> minutes at full power (with small containers of water in the oven chamber)
>
> did not affect the tertiary structure of the proteins in the tissues. Nor
> could I find a procedure in the lab or references as to where the idea
> originated. They are now being dried at 60 degrees celcius for one hour
> in
> an oven prior to staining. The pathologist's insisted that the problem
> was
> not with fixation but with staining, I made improvements there but as I've
>
> stated before I could not improve on the nuclear morphology. My question
> is,
> is this a fixation problem? Is this a slide drying problem as I have
> recently read on this site? I have read the article on alcohol/xylene
> tissue processing (Journal of Histotechnology March 2000 issue page 45,
> and
> the ideas seemed feasible but because it is unconventional, I would
> appreciate some other ideas and comments.
>
> Thanks for the input.
>
> Adrienne Vair MLT BMLSc
> Histology Supervisor
> Medicine Hat Regional Hospital
> ________________________________________________________________________
> Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
>
<< Previous Message | Next Message >>