Help!!!!!!!!! Embedding cells....urgent!!!

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From:Emma Carter <> (by way of Histonet)
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Sometimes, it would be really nice to have some notice of these things!!!!

Afetr i have severely castigated the relevant parties, please can someone

I wrote a while ago about embedding cells in paraffin.....Problem is,
collegue has started them growing today and is going to harvest on friday. I
am on holiday on friday afternoon, which means i have zero time on friday
morning to do anything with these cells.

So, i have loads of panic driven questions!!!

1) If i pellet them and put them into a gelatine capsule, will they be okay
in the fridge freezer over the weekend?
2) I cant get any histogel in time, so can someone let me have a suitable
recipe for gelatine???
3)Should i fix them in NBF or 4% Para?
4)Should i fix them before embedding them in gelatine?
5) Can i leave them in fixative over the weekend?
6) can i go home and cry!?

Cheers in advance!!!! I am in a major panic here......please reply soon!!!


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