HIER Methods Survey

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From:Luis Chiriboga <luis.chiriboga@med.nyu.edu>
To:Histonet <Histonet@pathology.swmed.edu>
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Dear Histonetters:
I am taking an informal survey to see what HIER methods/techniques
everyone is using.  As with any survey,  the more responses the better.
If anyone has additional questions they would like to add please e-mail
me directly and i will reissue a questionnaire.

1) Which heating method do you use for HIER? (i.e.. Microwave, pressure
cooker, combination etc...)
2) What is the average "real" heating time for antigen retrieval?
(actual boiling time)
4) How long is your cooling period?
5) Do you perform manual or automated IHC?
5) Do you prepare your own retrieval solutions or purchase commercial?
6) Do you use more than one HIER solution? (please list)
7) Which solution do you use the most?

I will post the stats as soon as I compile all the responses.
Thanks in advance

Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016

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