I'm Adam Link, an honours student in the zoology dapt at melbourne uni.
I'm attempting to stain bovine oocytes that are matured in vitro with the aim of identifying whether prophase 1 is reached or if the oocyte has matured to mataphase 2. Does anyone have any idea on stains or proceedures to use as i'm hoping to use potentially functioning oocytes / embryo's and would prefer not to fix them.
Many thanks
        Adam Link
 Adamlink@hotmail.com


BRUCE ABALOZ
HISTOLOGIST		      
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The UNIVERSITY of MELBOURNE        *  fax:   +61 3 93447909 
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