zebrafish

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From:Michelle Down <michellD@qimr.edu.au>
To:'HistoNet Server' <HistoNet@Pathology.swmed.edu>
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Hi all,
I was wondering what type of fixative people are using for zebrafish 
embryos, especially for whole mount in situ hybridisation? I have been 
using 4% paraformaldehyde in PBS and am getting a lot of wrinkly embryos. 
Any suggestions would be much appreciated.
Thanks,
Michelle



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