Vimentin and mouse

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From:"Coskran, Timothy M" <>
To:"Histonet (E-mail)" <>
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Does anyone have any experience with using the V9 vimentin clone on mouse
tissue?  We have had no luck getting any tissue that should be positive to
label at all.  The mouse tissues are formalin fixed for 24 hours.  We have
tried HIER, no antigen retrieval, and enzymatic methods. According to a
cross-reactivity chart from DAKO, this clone should label mouse vimentin.

Any help or suggestions would be appreciated.

Tim Coskran

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